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Changes of TLR-4, TNF-α, IL-6, and IL-17 after spinal tuberculosis surgery and their correlations with prognosis
XU Zuyuan, ZHONG Xin, PAN Jianchao, ZHANG Qiang
2024, 13 (1):
13-17.
doi: 10.3969/j.issn.2095-378X.2024.01.003
Objective To analyze the changes of Toll-like receptor (TLR)-4, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-17 after spinal tuberculosis surgery and their correlations with prognosis. Methods Sixty patients with spinal tuberculosis who received surgical treatment in our hospital from January 2021 to December 2022 were selected as the observation group, and 60 patients without spinal tuberculosis who underwent spinal surgery were selected as the control group (Some cases were from partner hospitals in the Fund). The expression levels of TLR-4, TNF-α, IL-6, and IL-17 in serum and lesion tissues of the two groups were detected. According to the prognosis 6 months after operation, patients in the observation group were divided into a good prognosis group and a poor prognosis group. Serum expression levels of TLR-4, TNF-α, IL-6, and IL-17 were compared between the two groups before and 6 months after operation. Pearson correlation was used to analyze the relationships between the expression levels of serum TLR-4, TNF-α, IL-6, and IL-17 before surgery and the score of modified Babbitt index (MBI) 6 months after surgery in patients with spinal tuberculosis. Receiver operating characteristic (ROC) curve was used to analyze the predictive efficacy of serum TLR-4, TNF-α, and IL-6 combined with IL-17 for postoperative poor prognosis of spinal tuberculosis. Results The expression levels of TLR-4, TNF-α, IL-6, and IL-17 in serum and lesion tissues of the observation group were higher than those of the control group before operation, with significant differences (P<0.05). The levels of TLR-4, TNF-α, IL-6, and IL-17 in the poor prognosis group were higher than those in the good prognosis group,with significant differences (P<0.05). Six months after surgery, the expression levels of TLR-4, TNF-α, IL-6, and IL-17 in serum of the good prognosis group were significantly lower than those before surgery, and the differences were statistically significant compared with those of the poor prognosis group (P<0.05). By Pearson correlation analysis, the expression levels of serum TLR-4,TNF-α, IL-6, and IL-17 in patients with spinal tuberculosis before surgery were negatively correlated with MBI scale score 6 months after surgery (P<0.05). According to ROC curve analysis, the area under curve of preoperative serum TLR-4, TNF-α, and IL-6 combined with IL-17 for predicting poor prognosis after spinal tuberculosis surgery was 0.921. Conclusion Serum TLR-4, TNF-α, IL-6, and IL-17 after spinal tuberculosis surgery are significantly lower than those before surgery, which is closely related to prognosis. Preoperative serum TLR-4, TNF-α, and IL-6 combined with IL-17 can predict poor prognosis effectively, which is worthy of clinical attention.
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